Overview
The NEB Tm Calculator is an essential tool for researchers in molecular biology to help determine the optimal annealing temperatures for PCR reactions. The melting temperature (Tm) is crucial for designing effective primers, impacting the efficiency of PCR. By inputting your primer sequences and their concentrations, this calculator provides a quick and efficient way to optimize your experiments.
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How to Use
- Enter your primer sequence: Make sure to input the correct nucleotide sequence (A, T, C, G). Any typos here could lead to unexpected results! 🧬
- Input the primer concentration: Specify the concentration in micromolar (uM). A valid input is necessary for accurate Tm calculations 💧.
- Hit the calculate button: With just a click, your results will appear below in a well-structured table! 📊
🔍 Remember: Accurate Tm calculations can significantly enhance your PCR success rates!
